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5e5 of NY-ESO-1 specific TCR-HEK293 cell line were stained with 100 μL of 1:25 dilution (4 μL stock solution in 100 μL FACS buffer) of APC-Labeled Human HLA-A*02:01&B2M&NY-ESO-1 (SLLMWITQV) Tetramer Protein (Cat. No. HL1-HA2H8) and negative control protein respectively. APC signal was used to evaluate the binding activity (QC tested).
Anti-NY-ESO-1 Antibody, Human IgG1 captured on Protein A Chip can bind Human HLA-A*02:01&B2M&NY-ESO-1 (SLLMWITQV) Tetramer Protein (Cat. No. HL1-H52C6) with an affinity constant of 0.752 nM as determined in a SPR assay (Biacore 8K) (QC tested).
The purity of Human HLA-A*02:01&B2M&NY-ESO-1 (SLLMWITQV) Tetramer Protein (Cat. No. HL1-H52C6) is more than 90% and the molecular weight of this protein is around 265-285 kDa verified by SEC-MALS.
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